TnSeq: Analysis and Applications of Sequencing Transposon insertion Mutant Libraries.
Thomas Ioerger, TAMU
Time: 9:00AM Monday March 20 2023 {US Central daylight saving time}
TnSeq, or sequencing of transposon insertion mutant libraries, is a
widely-used, high-throughput experimental methodology for profiling
the essentiality and conditional-essentiality of genes in bacterial
genomes. We will give a high-level summary of how this technology
works, and how it is used to uncover functional roles of genes and
their pathway associations. We will discuss some of the challenges in
statistical analysis of TnSeq data, which is subject to various
sources of noise, and some methods for quantifying the significance of
conditional effects on gene essentiality. Finally, we will survey
some results that have been obtained with TnSeq, and discuss
relationships to other 'omics methods.
Ioerger's Biography:
Thomas Ioerger is a Professor in the Computer Science and Engineering
Department at Texas A&M University. He earned his BS degree in
Molecular and Cell Biology at the Pennsylvania State University in
1989, and his PhD in Computer Science from the University of Illinois
in 1996. His research interests include AI, Machine Learning,
Bioinformatics, and Genomics, with a focus on applications to
understanding antibiotic mechanisms of action, evolution of drug
resistance, and novel drug discovery for tuberculosis and other
infectious diseases. He has published over 200 papers in
peer-reviewed journals and conferences. His research group develops
and distributes Transit, a python-based software system for
statistical analysis of TnSeq data. He has received research funding
from NIH, NSF, CPRIT (Cancer Research and Prevention Institute of
Texas), and the Bill & Melinda Gates Foundation.
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